Investigation of apigenin-7-O-β-glucoside hydrolysis by β-glucosidase from almonds

Abstract

The enzymatic transformation of apigenin-7-O-β-glucoside into apigenin is effected using β-glucosidase from almonds. Kinetic studies gave the following values Km=7.63×10-4mol/dm3, vmax=10.37 μmol mg-1 min-1 and E1=65.21 kJ/mol when the substrate was pure apigenin-7-O-β-glucoside, while in case of apigenin-7-O-β-glucoside from dry extract of camomile ligulate flowers E2 = 65.57 kJ/mol. © 1994 Lonsdale Press Ltd.