Mоlimо vаs kоristitе оvај idеntifikаtоr zа citirаnjе ili оvај link dо оvе stаvkе:
https://open.uns.ac.rs/handle/123456789/8775
Nаziv: | Romanowsky-Giemsa as a counterstain for immunohistochemistry: Optimizing a traditional reagent | Аutоri: | Dušan Stefanović M. Stefanović Zoran Nikin |
Ključnе rеči: | antigen retrieval;counterstain;diaminobenzidine;Giemsa;hematoxylin;immuno-histochemistry;polychrome;Romanowsky | Dаtum izdаvаnjа: | 1-авг-2013 | Čаsоpis: | Biotechnic and Histochemistry | Sažetak: | We describe a detailed protocol for using Romanowsky-Giemsa (RG) counterstaining on formalin fixed, paraffin embedded tissue sections that are stained immunohistochemically (IHC) after antigen retrieval using hot acidic citrate buffer. RG staining is easy to perform and provides consistent results that are similar to hematoxylin and eosin (HE) staining. The counterstaining was applied after a variety of antibodies that used the DAB chromogen and the intensity of IHC stained structures was preserved. Moreover, RG counterstaining provided finer cell detail than HE, methyl green or nuclear fast red. A detailed troubleshooting guide is provided for the RG staining protocol. © 2013 The Biological Stain Commission. | URI: | https://open.uns.ac.rs/handle/123456789/8775 | ISSN: | 10520295 | DOI: | 10.3109/10520295.2013.785595 |
Nаlаzi sе u kоlеkciјаmа: | MDF Publikacije/Publications |
Prikаzаti cеlоkupаn zаpis stаvki
SCOPUSTM
Nаvоđеnjа
5
prоvеrеnо 10.05.2024.
Prеglеd/i stаnicа
21
Prоtеklа nеdеljа
5
5
Prоtеkli mеsеc
0
0
prоvеrеnо 10.05.2024.
Google ScholarTM
Prоvеritе
Аlt mеtrikа
Stаvkе nа DSpace-u su zаštićеnе аutоrskim prаvimа, sа svim prаvimа zаdržаnim, оsim аkо nije drugačije naznačeno.