Please use this identifier to cite or link to this item: https://open.uns.ac.rs/handle/123456789/8775
DC FieldValueLanguage
dc.contributor.authorDušan Stefanovićen_US
dc.contributor.authorM. Stefanovićen_US
dc.contributor.authorZoran Nikinen_US
dc.date.accessioned2019-09-30T09:11:05Z-
dc.date.available2019-09-30T09:11:05Z-
dc.date.issued2013-08-01-
dc.identifier.issn10520295en_US
dc.identifier.urihttps://open.uns.ac.rs/handle/123456789/8775-
dc.description.abstractWe describe a detailed protocol for using Romanowsky-Giemsa (RG) counterstaining on formalin fixed, paraffin embedded tissue sections that are stained immunohistochemically (IHC) after antigen retrieval using hot acidic citrate buffer. RG staining is easy to perform and provides consistent results that are similar to hematoxylin and eosin (HE) staining. The counterstaining was applied after a variety of antibodies that used the DAB chromogen and the intensity of IHC stained structures was preserved. Moreover, RG counterstaining provided finer cell detail than HE, methyl green or nuclear fast red. A detailed troubleshooting guide is provided for the RG staining protocol. © 2013 The Biological Stain Commission.en_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnic and Histochemistryen_US
dc.subjectantigen retrievalen_US
dc.subjectcounterstainen_US
dc.subjectdiaminobenzidineen_US
dc.subjectGiemsaen_US
dc.subjecthematoxylinen_US
dc.subjectimmuno-histochemistryen_US
dc.subjectpolychromeen_US
dc.subjectRomanowskyen_US
dc.titleRomanowsky-Giemsa as a counterstain for immunohistochemistry: Optimizing a traditional reagenten_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.doi10.3109/10520295.2013.785595-
dc.identifier.pmid88-
dc.identifier.scopus2-s2.0-84880383019-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84880383019-
dc.description.versionPublisheden_US
dc.relation.lastpage335en_US
dc.relation.firstpage329en_US
dc.relation.issue6en_US
dc.relation.volume88en_US
item.grantfulltextnone-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0002-5796-8296 -
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