Romanowsky-Giemsa as a counterstain for immunohistochemistry: Optimizing a traditional reagent

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Назив:	Romanowsky-Giemsa as a counterstain for immunohistochemistry: Optimizing a traditional reagent
Аутори:	Dušan Stefanović M. Stefanović Zoran Nikin
Кључне речи:	antigen retrieval;counterstain;diaminobenzidine;Giemsa;hematoxylin;immuno-histochemistry;polychrome;Romanowsky
Датум издавања:	1-авг-2013
Часопис:	Biotechnic and Histochemistry
Сажетак:	We describe a detailed protocol for using Romanowsky-Giemsa (RG) counterstaining on formalin fixed, paraffin embedded tissue sections that are stained immunohistochemically (IHC) after antigen retrieval using hot acidic citrate buffer. RG staining is easy to perform and provides consistent results that are similar to hematoxylin and eosin (HE) staining. The counterstaining was applied after a variety of antibodies that used the DAB chromogen and the intensity of IHC stained structures was preserved. Moreover, RG counterstaining provided finer cell detail than HE, methyl green or nuclear fast red. A detailed troubleshooting guide is provided for the RG staining protocol. © 2013 The Biological Stain Commission.
URI:	https://open.uns.ac.rs/handle/123456789/8775
ISSN:	10520295
DOI:	10.3109/10520295.2013.785595
Налази се у колекцијама:	MDF Publikacije/Publications