Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/32634
Title: | Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing | Authors: | Mozhgan Keshavarz Parvin Alizadeh Firoz Babu Kadumudi Gorka Orive Akhilesh K. Gaharwar Miguel Castilho Nasim Golafshan Alireza Dolatshahi-Pirouz |
Keywords: | alginate;laponite;hydrogels;mesenchymal stem cells;nanosilicate | Issue Date: | 19-Apr-2023 | Publisher: | American Chemical Society | Project: | Twinning for reaching sustainable scientific and technological excellence in the field of Green Electronics (GREENELIT) | Journal: | ACS Applied Materials and Interfaces | Abstract: | Several studies have shown that nanosilicate-reinforced scaffolds are suitable for bone regeneration. However, hydrogels are inherently too soft for load-bearing bone defects of critical sizes, and hard scaffolds typically do not provide a suitable three-dimensional (3D) microenvironment for cells to thrive, grow, and differentiate naturally. In this study, we bypass these longstanding challenges by fabricating a cell-free multi-level implant consisting of a porous and hard bone-like framework capable of providing load-bearing support and a softer native-like phase that has been reinforced with nanosilicates. The system was tested with rat bone marrow mesenchymal stem cells in vitro and as a cell-free system in a critical-sized rat bone defect. Overall, our combinatorial and multi-level implant design displayed remarkable osteoconductivity in vitro without differentiation factors, expressing significant levels of osteogenic markers compared to unmodified groups. Moreover, after 8 weeks of implantation, histological and immunohistochemical assays indicated that the cell-free scaffolds enhanced bone repair up to approximately 84% following a near-complete defect healing. Overall, our results suggest that the proposed nanosilicate bioceramic implant could herald a new age in the field of orthopedics. | URI: | https://open.uns.ac.rs/handle/123456789/32634 | ISSN: | 1944-8244 | DOI: | https://doi.org/10.1021/acsami.3c01717 | Rights: | Attribution-NonCommercial-NoDerivs 3.0 United States |
Appears in Collections: | Projekti |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
keshavarz-et-al-2023-multi-leveled-nanosilicate-implants-can-facilitate-near-perfect-bone-healing.pdf | 3.26 MB | Adobe PDF | View/Open |
Page view(s)
18
Last Week
13
13
Last month
0
0
checked on May 3, 2024
Download(s)
1
checked on May 3, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License