Improved thin-layer chromatography separation of carbohydrates in wort and beer

Abstract

With a slight modification the method previously reported by Vrbaški and Lepojević, J. Chromatog. 558, 328-332 (1991) can be applied to the analysis of carbohydrates in worts, beer and brewing syrup. The modification introduces new a third step to the development of plates using chloroform/glac. acetic acid/water (2;6;2 by vol). By this method it is possible to separate maltooligosaccharides up to 17 spots. Evidence of a difference between the yeast strains in fermentation of carbohydrate is also presented. © 1992 Science & Technology Letters.