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Title: | Study of association of 12-Monoketocholic acid by <sup>1</sup>H NMR relaxation method | Authors: | Pósa M. Guzvanj (Guzsvány), V. Csanádi J. Borbás J. Gaál F. |
Issue Date: | 1-Dec-2009 | Journal: | Acta Chimica Slovenica | Abstract: | 12-Monoketocholic acid (12-MKC) shows a promotive effect in the transport of some drugs (lidocaine, morphine, quinine, gliclazide, etc.). In the mechanism of pharmacological action of 12-MKC an important role plays its aggregation (self-association), as well as formation of mixed micelles. These phenomena were studied by the 1H NMR spin-lattice relaxation method using solutions of sodium salt of 12-MKC in D2O and in D2O solution saturated with 1-octanol. The function describing the dependence of the spin-lattice relaxation time (T1) on the concentration of Na-12-MKC (cBA) in D2O has one inflexion point (at the concentration of 48 mM), whereas the same function for the saturated solution of 1-octanol has two inflexion points (one at the concentration of 45 mM and the other at 87 mM). These differences in the curves T1 = f(cBA), as well as the shift of the solubilization curve of lecithin in the presence of 1-octanol towards higher concentrations of Na-12-MKC, indicate that sodium salt of this keto-bile acid forms mixed micelles with 1-ocatnol, so that the application of the micellar solution of Na-12-MKC in the presence of 1-octanol decreases membrane toxicity (membrane lysis) of this bile acid. The curves T1 = f(cBA) for methyl esters of 12-MKC and cholic acid in the CDCl3 solution have one inflexion point at the concentration above 5 mM, indicating formation of the micelles of Small's type, whereas methyl ester of 3,7,12-triketocholic acid forms a gel-like structure. | URI: | https://open.uns.ac.rs/handle/123456789/9760 | ISSN: | 13180207 |
Appears in Collections: | PMF Publikacije/Publications |
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