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https://open.uns.ac.rs/handle/123456789/932
Title: | Multidirectional approaches on autofermented chamomile ligulate flowers: Antioxidant, antimicrobial, cytotoxic and enzyme inhibitory effects | Authors: | Cvetanović, Aleksandra Zeković, Zoran Zengin, Gokhan Mašković, Pavle Petronijević, Mirjana Radojković, Marija |
Issue Date: | 1-Jan-2019 | Journal: | South African Journal of Botany | Abstract: | © 2018 South African Association of Botanists In the frame of the present paper the enzymatic transformation of apigenin-glucosides into free aglycone was achieved by autofermentation of chamomile ligulate flowers (CLF). Antioxidant properties of the autofermented CLF (A-CLF) extract were evaluated by their radical scavenging activity against hydroxyl radicals and inhibition of lipid peroxidation. Obtained results showed that A-CLF extract in a concentration of 0.84 mg/mL was able to inhibit 50% of hydroxyl radicals, while IC 50 value in the case of inhibition of lipid peroxidation was 5.21 mg/mL. Antimicrobial activity was done by measuring minimal inhibitory concentration (MIC) values for eight microbial strains. Obtained MIC values (9.75–156.25 μg/mL) confirmed high antibacterial and antifungal activities of the extract. Cytotoxic activity was done by using three histological different cell lines: Hep2C; RD and L2OB. Obtained IC 50 values for these cell lines were: 28.72; 17.31 and 10.92, respectively. Furthermore, in vitro investigation of the A-CLF ability to inhibit selected enzymes (α-amylase, α-glucosidase, tyrosinase) was done as well. Determined activities against α-amylase and α-glucosidase were 0.94 and 3.24 mmol ACAE/g, respectively. Further, measured activity against tyrosinase was 0.69 mg KAEs/g indicating high enzyme-inhibitory activity of examined sample. Results demonstrated that A-CLF extracts showed considerable pharmacological activity. | URI: | https://open.uns.ac.rs/handle/123456789/932 | ISSN: | 02546299 | DOI: | 10.1016/j.sajb.2018.01.003 |
Appears in Collections: | TF Publikacije/Publications |
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