Determination of ionophore coccidiostat salinomycine in premixes and poultry feeding stuffs by liquid chromatography after post-column derivatisation

Abstract

© 2014, Association of Chemists and Chemical Engineers of Serbia. All rights reserved. Coccidiosis is a common parasitic disease of broiler chickens caused by singlecelled protozoan parasites of the genus Eimeria, which are commonly referred toas “coccidian”. This is an infective disease of the digestive tract which is most frequent amongpoultry, causing a decrease in daily increment, prolonged fattening, poorer skin pigmentation, slower feed conversion and increased mortality. The disease is caused by Protozoas from the genera of Eimeria, Isospora and Cryptospora, and it is manifested by damaging the intestine epithelial cells, less frequently the bile duct and renaltubuli. Coccidiosis is traditionally controlled by chemotherapy. There are many anticoccidial preparations which are used in the prevention of coccidiosis. Wechose a polyether monocarboxylic acid – salinomycine. Salinomycine consists monovalent carboxyl–polyether ionophores. Salinomycine, produced by Streptomyces albus, destroys the cell membranes and causes their lysis. Salinomycineand other ionophoric antibiotics combined with a number of mono and divalent cations and in the form of bi-complexes are capable to transfer metal ionsthrough lipid hydrophobic membrane, and when they areadded to diet, they change bioavailability, gut uptake andabsorption and reserves of nutrient tissues. The validationprocess of liquid chromatography determination of ionophoriccoccidiostatsalinomycine with UV spectrophotometric detection and post-column derivatisation with dimethylaminobenzaldehyde (DMAB) has been developed in this paper. The method is based on extraction of salinomycine in animal feed samples by using the mixture of acetonitrile and water (80:20, V/V) and by purification of extracts obtained by the filter 0.2 μm Acrodisc®PSF. The relative standard deviation (RSD) for reproducibility and accuracy varied from 2.4 to 8.8% and from 2.6 to 8.8%, respectively, and the values for the relative recovery rate ranged from 89 to 98%. The limit of detection (LOD) and limit of quantification (LOQ) were estimated to be below 47 and 71 μg/kg, respectively. Based on theseresults, it is concluded that the described method is accurate, precise, selective and reproducible and can be applied for determination of salinomycine in feeds and premixes for poultry.