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https://open.uns.ac.rs/handle/123456789/32746
Nаziv: | Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes | Аutоri: | Miler, Irena Rabasović, Mihailo Stylianou, Andreas Korać, Bato Korać, Aleksandra |
Ključnе rеči: | dermal collagen I; diabetes; l-arginine; polarization-resolved SHG imaging; Raman spectromicroscopy; AFM | Dаtum izdаvаnjа: | апр-2024 | Čаsоpis: | Bioengineering | Sažetak: | Changes in the structural properties of the skin due to collagen alterations are an important factor in diabetic skin complications. Using a combination of photonic methods as an optic diagnostic tool, we investigated the structural alteration in rat dermal collagen I in diabetes, and after short-term l-arginine treatment. The multiplex approach shows that in the early phase of diabetes, collagen fibers are partially damaged, resulting in the heterogeneity of fibers, e.g., “patchy patterns” of highly ordered/disordered fibers, while l-arginine treatment counteracts to some extent the conformational changes in collagen-induced by diabetes and mitigates the damage. Raman spectroscopy shows intense collagen conformational changes via amides I and II in diabetes, suggesting that diabetes-induced structural changes in collagen originate predominantly from individual collagen molecules rather than supramolecular structures. There is a clear increase in the amounts of newly synthesized proline and hydroxyproline after treatment with l-arginine, reflecting the changed collagen content. This suggests that it might be useful for treating and stopping collagen damage early on in diabetic skin. Our results demonstrate that l-arginine attenuates the early collagen I alteration caused by diabetes and that it could be used to treat and prevent collagen damage in diabetic skin at a very early stage. | URI: | https://open.uns.ac.rs/handle/123456789/32746 | ISSN: | 2306-5354 | DOI: | 10.3390/bioengineering11040407 |
Nаlаzi sе u kоlеkciјаmа: | IBS Publikacije/Publications |
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bioengineering-11-00407-v2.pdf | 2.45 MB | Adobe PDF | Pоglеdајtе |
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