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https://open.uns.ac.rs/handle/123456789/2044
Title: | L-lysine determination in animal feed using microbiological microtiter plate assay | Authors: | Jakšić, S. Jajić, Igor Krstović, Saša Mihaljev |
Issue Date: | 1-Jan-2018 | Journal: | Journal of the Hellenic Veterinary Medical Society | Abstract: | © 2018 Hellenic Veterinary Medical Society.All Rights Reserved. Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required. | URI: | https://open.uns.ac.rs/handle/123456789/2044 | ISSN: | 17922720 | DOI: | 10.12681/jhvms.18881 |
Appears in Collections: | POLJF Publikacije/Publications |
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