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https://open.uns.ac.rs/handle/123456789/2044
Pоljе DC-а | Vrеdnоst | Јеzik |
---|---|---|
dc.contributor.author | Jakšić, S. | en_US |
dc.contributor.author | Jajić, Igor | en_US |
dc.contributor.author | Krstović, Saša | en_US |
dc.contributor.author | Mihaljev | en_US |
dc.date.accessioned | 2019-09-23T10:19:15Z | - |
dc.date.available | 2019-09-23T10:19:15Z | - |
dc.date.issued | 2018-01-01 | - |
dc.identifier.issn | 17922720 | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/2044 | - |
dc.description.abstract | © 2018 Hellenic Veterinary Medical Society.All Rights Reserved. Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required. | en |
dc.relation.ispartof | Journal of the Hellenic Veterinary Medical Society | en |
dc.title | L-lysine determination in animal feed using microbiological microtiter plate assay | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.doi | 10.12681/jhvms.18881 | - |
dc.identifier.scopus | 2-s2.0-85057450232 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85057450232 | - |
dc.description.version | Unknown | en_US |
dc.relation.lastpage | 1100 | en |
dc.relation.firstpage | 1094 | en |
dc.relation.issue | 3 | en |
dc.relation.volume | 69 | en |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Departman za stočarstvo | - |
crisitem.author.dept | Departman za stočarstvo | - |
crisitem.author.orcid | 0000-0003-1235-8686 | - |
crisitem.author.orcid | 0000-0001-9347-4886 | - |
crisitem.author.parentorg | Poljoprivredni fakultet | - |
crisitem.author.parentorg | Poljoprivredni fakultet | - |
Nаlаzi sе u kоlеkciјаmа: | POLJF Publikacije/Publications |
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