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https://open.uns.ac.rs/handle/123456789/19148
Назив: | Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine | Аутори: | Jovanov, Pavle Vraneš, Milan Sakač, Marijana Gadžurić, Slobodan Panić, Jovana Marić, Aleksandar Ostojić, Sergej |
Датум издавања: | 22-окт-2018 | Часопис: | Analytica Chimica Acta | Сажетак: | The lack of one or the other may compromise their role in the energy transport and cell signaling. A reliable estimate of their levels in biological samples is imperative since they are important markers of many metabolic disorders. Therefore, a new LC-MS/MS method for simultaneous determination and quantification of GAA and Cr by multiple reaction monitoring (MRM) mode was developed based on the hydrophilic interaction chromatography (HILIC) and response surface methodology (RSM) for the optimization of chromatographic parameters. The optimized parameters ensured good separation of these similar, very polar molecules (chromatographic resolution > 1.5) without prior derivatization step in a short analysis run (6 min). The developed method was validated to ensure accurate (R, 75.1–101.6%), precise (RSD < 20%) and low quantification (LOQ of 0.025 μg mL−1 for GAA and 0.006 μg mL−1 for Cr) of the tested analytes and the use of matrix-matched calibration eliminated variable effects of complex matrices such as human plasma and urine. Therefore, this method can be implemented in medical laboratories as a tool for the diagnostics of creatine deficiencies and monitoring of guanidinoacetate and creatine supplementation regimes in biological samples. | URI: | https://open.uns.ac.rs/handle/123456789/19148 | ISSN: | 0003-2670 | DOI: | 10.1016/j.aca.2018.03.038 (BISIS)109211 (BISIS)109211 |
Налази се у колекцијама: | FSFV Publikacije/Publications FINS Publikacije/Publications |
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