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https://open.uns.ac.rs/handle/123456789/13985
Title: | Enzymatic transformation of desacetyl-lanatoside A into digitoxin by β-glucosidase action in cyclodextrin solutions | Authors: | Pekić B. Lepojević Z. |
Issue Date: | 1-Jun-1991 | Journal: | Biotechnology Letters | Abstract: | The enzymatic transformation of desacetyl-lanatoside A (DLA) to its secondary glycoside, digitoxin, in solutions of β-and γ-cyclodextrins is effected using of β-glucosidase from barley. Due to the interaction of cyclodextrins (CyDs) with desacetyl-lanatoside A, an increase in solubility of the latter of 24.5 and 230 times was observed for β-cyclodextrin and γ-cyclodextrin, respectively. Kinetic studies of the enzymatic transformation gave for β-glucosidase the values KM=3.3×10-4 mol. dm-3 and Vmax=0.557 μmol mg-1 min-1 when the substrate was the deacetyl-lanatoside A complex with β-cyclodextrin, while in the case of the complex with γ-cyclodextrin these values were KM=5.45×10-4 mol dm-3 and Vmax=0.896 μmol mg-1 min-1. © 1991 Science and Technology Letters. | URI: | https://open.uns.ac.rs/handle/123456789/13985 | ISSN: | 01415492 | DOI: | 10.1007/BF01030990 |
Appears in Collections: | TF Publikacije/Publications |
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