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https://open.uns.ac.rs/handle/123456789/13709
Nаziv: | Expression analysis of genes involved in epigenetic regulation and apoptosis in human malignant haematopoietic cell lines treated with 5-azacytidine | Аutоri: | Karmen Stankov Gordana Bogdanović Vesna Kojić Sunčica Stankov Njegica Jojić Luka Popović Stevan Popović |
Ključnе rеči: | genes expression;haematopoietic cell lines;5-azacytidine | Dаtum izdаvаnjа: | 1-јан-2011 | Čаsоpis: | Journal of B.U.ON. | Sažetak: | Purpose: The aim of this study was to investigate the modulation ofthe expression status of 10 different genes involved in epigenetic regulation and apoptosis by the DNA methyltransferase (DNMT) inhibitor 5-azacytidine (5-Aza), as markers of response to treatment, in two different human malignant haematopoietic cell lines. Methods: In our analysis we used the SybrGreen technology and gene-specific primers for the qRT-PCR analysis of 10 genes, in cDNA of PC-MDS and K562 cell lines, treated by 1 micromole of 5-Aza for 24h. Results: DNMT1 and DNMT3A showed statistically significant decrease of expression in 5-Aza-treated PC-MDS cells, whereas DNMT3B showed significantly decreased expression in 5-Aza-treated K562 cells. The members of the Bcl-2 family of apoptosis-regulating genes Bcl-2 and Bax showed statistically significant differences in expression, in comparison with non-treated PC-MDS cells. Our most interesting result was the significant upregulation (re-expression) of p15, in 5-Aza-treated PC-MDS cells. Conclusion: The re-expression of p15 in PC-MDS cell line evaluated by qRT-PCR makes this novel cell line a suitable model for the studies of pharmacologic demethylation as a plausible mechanism resulting in hematologic response in myelodysplastic syndrome (MDS). © 2011 Zerbinis Medical Publications. | URI: | https://open.uns.ac.rs/handle/123456789/13709 | ISSN: | 11070625 |
Nаlаzi sе u kоlеkciјаmа: | MDF Publikacije/Publications |
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