Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/13709
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Karmen Stankov | en_US |
dc.contributor.author | Gordana Bogdanović | en_US |
dc.contributor.author | Vesna Kojić | en_US |
dc.contributor.author | Sunčica Stankov | en_US |
dc.contributor.author | Njegica Jojić | en_US |
dc.contributor.author | Luka Popović | en_US |
dc.contributor.author | Stevan Popović | en_US |
dc.date.accessioned | 2020-03-03T14:53:25Z | - |
dc.date.available | 2020-03-03T14:53:25Z | - |
dc.date.issued | 2011-01-01 | - |
dc.identifier.issn | 11070625 | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/13709 | - |
dc.description.abstract | Purpose: The aim of this study was to investigate the modulation ofthe expression status of 10 different genes involved in epigenetic regulation and apoptosis by the DNA methyltransferase (DNMT) inhibitor 5-azacytidine (5-Aza), as markers of response to treatment, in two different human malignant haematopoietic cell lines. Methods: In our analysis we used the SybrGreen technology and gene-specific primers for the qRT-PCR analysis of 10 genes, in cDNA of PC-MDS and K562 cell lines, treated by 1 micromole of 5-Aza for 24h. Results: DNMT1 and DNMT3A showed statistically significant decrease of expression in 5-Aza-treated PC-MDS cells, whereas DNMT3B showed significantly decreased expression in 5-Aza-treated K562 cells. The members of the Bcl-2 family of apoptosis-regulating genes Bcl-2 and Bax showed statistically significant differences in expression, in comparison with non-treated PC-MDS cells. Our most interesting result was the significant upregulation (re-expression) of p15, in 5-Aza-treated PC-MDS cells. Conclusion: The re-expression of p15 in PC-MDS cell line evaluated by qRT-PCR makes this novel cell line a suitable model for the studies of pharmacologic demethylation as a plausible mechanism resulting in hematologic response in myelodysplastic syndrome (MDS). © 2011 Zerbinis Medical Publications. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Journal of B.U.ON. | en_US |
dc.subject | genes expression | en_US |
dc.subject | haematopoietic cell lines | en_US |
dc.subject | 5-azacytidine | en_US |
dc.title | Expression analysis of genes involved in epigenetic regulation and apoptosis in human malignant haematopoietic cell lines treated with 5-azacytidine | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.pmid | 16 | - |
dc.identifier.scopus | 2-s2.0-79954473227 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/79954473227 | - |
dc.description.version | Published | en_US |
dc.relation.lastpage | 122 | en_US |
dc.relation.firstpage | 116 | en_US |
dc.relation.issue | 1 | en_US |
dc.relation.volume | 16 | en_US |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Medicinski fakultet, Katedra za biohemiju | - |
crisitem.author.dept | Medicinski fakultet | - |
crisitem.author.orcid | 0000-0002-3888-3473 | - |
crisitem.author.parentorg | Medicinski fakultet | - |
crisitem.author.parentorg | Univerzitet u Novom Sadu | - |
Appears in Collections: | MDF Publikacije/Publications |
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