Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/12854
Title: | Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid | Authors: | Radosavljević M. Lević S. Belović, Miona Pejin, Jelena Djukić-Vuković A. Mojović L. Nedović V. |
Issue Date: | 1-Jan-2019 | Journal: | Bioprocess and Biosystems Engineering | Abstract: | © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability, L. rhamnosus cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g−1) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L−1 h−1, respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L−1 h−1. This study suggested that the “freezing–thawing” technique can be successfully used for immobilization of L. rhamnosus in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability. | URI: | https://open.uns.ac.rs/handle/123456789/12854 | ISSN: | 16157591 | DOI: | 10.1007/s00449-019-02228-0 |
Appears in Collections: | FINS Publikacije/Publications |
Show full item record
SCOPUSTM
Citations
19
checked on Apr 29, 2023
Page view(s)
38
Last Week
16
16
Last month
1
1
checked on May 10, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.