High-performance liquid chromatography of some digitalis glycosides and their aglycones on chemically bonded phases

Abstract

The retention behaviour of some glycosides their aglycones was studied as a function of the composition of the acetonitrile-water eluent. The relationship between the retention constant (log k′) and the volume fraction of acetonitrile in the eluent was parabolic and of shape indicating a "dual" retention mechanism for all solutes studied. This behaviour is discussed in terms of the nature of the solute, eluent and stationary phase. © 1983.