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https://open.uns.ac.rs/handle/123456789/9860
Title: | Bromocriptine-induced inhibition of hydroxylase/lyase activity of adult rat Leydig cells | Authors: | Kovačević, Radmila Sarač, Miroslav |
Issue Date: | 1-Jan-1993 | Journal: | Journal of Steroid Biochemistry and Molecular Biology | Abstract: | The present in vitro studies using a suspension of Leydig cells from adult rat testis demonstrated that bromocriptine (BR, 2 × 10-5M) inhibits hCG-stimulated testosterone production (in the presence of submaximal and maximal doses of hCG), while basal production was unaffected. When the cells were exposed to 8-bromo-cAMP either in the presence or absence of hCG, the inhibitory effect of BR was not reversed. In intact cells, BR inhibited conversion of progesterone and 17-hydroxy-progesterone to testosterone while conversion of androstenedione was not affected. Incubation of homogenates of Leydig cells in the presence of limiting NADPH concentrations (≤ 0.1 mM) resulted in significant BR-induced inhibition of conversion of progesterone (10 μM) to testosterone, while in the presence of "high" concentrations of NADPH (≥ 0.5 mM) BR was without effect. Present results suggest that BR inhibits androgen production at the level of the microsomal enzymes 17α-hydroxylase and/or 17,20-lyase. The inhibitory effect of BR using homogenates of Leydig cells was evident only in the presence of limiting NADPH concentrations that suggests a competitive-like pattern of inhibition, but mechanisms by which BR decreases activity of microsomal enzymes remain to be determined. © 1993. | URI: | https://open.uns.ac.rs/handle/123456789/9860 | ISSN: | 09600760 | DOI: | 10.1016/0960-0760(93)90327-S |
Appears in Collections: | Naučne i umetničke publikacije |
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