Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/9860
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kovačević, Radmila | en_US |
dc.contributor.author | Sarač, Miroslav | en_US |
dc.date.accessioned | 2020-03-03T14:35:27Z | - |
dc.date.available | 2020-03-03T14:35:27Z | - |
dc.date.issued | 1993-01-01 | - |
dc.identifier.issn | 09600760 | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/9860 | - |
dc.description.abstract | The present in vitro studies using a suspension of Leydig cells from adult rat testis demonstrated that bromocriptine (BR, 2 × 10-5M) inhibits hCG-stimulated testosterone production (in the presence of submaximal and maximal doses of hCG), while basal production was unaffected. When the cells were exposed to 8-bromo-cAMP either in the presence or absence of hCG, the inhibitory effect of BR was not reversed. In intact cells, BR inhibited conversion of progesterone and 17-hydroxy-progesterone to testosterone while conversion of androstenedione was not affected. Incubation of homogenates of Leydig cells in the presence of limiting NADPH concentrations (≤ 0.1 mM) resulted in significant BR-induced inhibition of conversion of progesterone (10 μM) to testosterone, while in the presence of "high" concentrations of NADPH (≥ 0.5 mM) BR was without effect. Present results suggest that BR inhibits androgen production at the level of the microsomal enzymes 17α-hydroxylase and/or 17,20-lyase. The inhibitory effect of BR using homogenates of Leydig cells was evident only in the presence of limiting NADPH concentrations that suggests a competitive-like pattern of inhibition, but mechanisms by which BR decreases activity of microsomal enzymes remain to be determined. © 1993. | en |
dc.relation.ispartof | Journal of Steroid Biochemistry and Molecular Biology | en |
dc.title | Bromocriptine-induced inhibition of hydroxylase/lyase activity of adult rat Leydig cells | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.doi | 10.1016/0960-0760(93)90327-S | - |
dc.identifier.scopus | 2-s2.0-0027761879 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/0027761879 | - |
dc.description.version | Unknown | en_US |
dc.relation.lastpage | 845 | en |
dc.relation.firstpage | 841 | en |
dc.relation.issue | 6 | en |
dc.relation.volume | 46 | en |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Naučne i umetničke publikacije |
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