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https://open.uns.ac.rs/handle/123456789/8806
Nаziv: | Antifungal activity of quinhydrone against Saccharomyces cerevisiae | Аutоri: | Ćurčić, Nataša Ranitović (Velićanski), Aleksandra Cvetković, Dragoljub Morina, Filis Veljović-Jovanović, Sonja Panković, Dejana |
Dаtum izdаvаnjа: | 17-јул-2013 | Izdаvаč: | PARLAR SCIENTIFIC PUBLICATIONS (P S P), FREISING | Čаsоpis: | Fresenius Environmental Bulletin | Sažetak: | Quinhydrone (QH) is a redox active charge transfer complex commonly used as a redox standard. Information on quinhydrone generation in plants is scarce and its physiological role is still unclear. Recently we have showed that excess zinc may induce oxidative stress through QH accumulation in the cell wall and stabilization of phenoxyl radicals [1]. The aim of our research was to investigate the antifungal activity of quinhydrone against yeast Saccharomyces cerevisiae (112, Hefebank Weihenstephan). Saccharomyces cerevisiae was grown on the Sabouraud maltose broth (HiMedia, Mumbai, India) in the presence of different concentrations of QH ranging from 75 μM to 500 μM. Concentrations of QH greater than 300 fiM had complete inhibitory effect on yeast growth, while lower concentrations (up to 200 μM) did not affect the growth. QH had a significant impact on antioxidative defense enzymatic systems, indicated by the changes in the activity in catalase (CAT) and superoxide dismutase (SOD). CAT activities increased by 43% (150 nM QH) and SOD activities by 122% and 60% at 150 jiM and 220μM QH respectively. However, abrupt inhibition of both enzymes was observed at concentrations higher than 220 μM QH (≥70%), to be almost completely diminished at 280 μM QH. High molecular weight genomic DNA without any laddering or smearing was detected in both control and QH treated yeast cells, indicating the absence of apoptosis. | URI: | https://open.uns.ac.rs/handle/123456789/8806 | ISSN: | 10184619 |
Nаlаzi sе u kоlеkciјаmа: | TF Publikacije/Publications |
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prоvеrеnо 10.05.2024.
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