Thermodynamic study of Hydrangea aspera chlorophyll catabolites by reverse phase liquid chromatography

Abstract

The Hydrangea aspera chlorophyll catabolites present in autumnal leaves were investigated. The thermodynamic study of the Hydrangea aspera chlorophyll catabolites was done using reversed phase liquid chromatography on the C4 and C8 analytical columns with water (acidified):methanol mobile phase in combination with ultraviolet detection and electrospray ionization mass spectrometry identification. The retention behaviors of Hydrangea aspera chlorophyll catabolites over a temperature range of 278-318 K were investigated. The data obtained permitted the construction of the van't Hoff plots. The stationary phase composition influences the thermodynamic retention of the Hydrangea aspera chlorophyll catabolites.