Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/7673
Title: | Usage of different molecular markers in delimitation of cryptic taxa in merodon avidus species complex (Diptera: Syrphidae) | Authors: | Popović, Dunja Đan, Mihajla Šašić Zorić, Ljiljana Šnjegota, Dragana Obreht, Dragana Vujić, Ante |
Issue Date: | 1-Jan-2014 | Journal: | Acta Zoologica Bulgarica | Abstract: | Three populations of Merodon avidus species complex from two geographical areas in the Danube River region (Djerdap and Fruska Gora) were used in this study with the aim to delimitate cryptic taxa within the complex. According to the diagnostic morphological characters, as well as to the season of their adult activity, samples were presumably identified as M. avidus Rossi or M. moenium Wiedemann. In order to reveal genetic differentiation between these taxa and to evaluate their current taxonomic status, two different types of molecular markers were used, allozymes and mtDNA. The analysis of 5 enzyme loci revealed the clear presence of two separate taxa, with presence of unique alleles in AAT (EC number 2.6.1.1) and ME (EC number 1.1.1.40) loci in M. avidus and M. moenium populations. The UPGMA dendogram based on Nei's genetic distance showed the presence of M. avidus and M. moenium cluster. On the other hand, the parsimony tree based on cytochrome c oxidase subunit I (COI) mtDNA sequences failed to discriminate these two taxa. The haplotype analysis revealed that one haplotype was shared between M. avidus and M. moenium. This led us to the conclusion that the allozyme data with species-specific alleles are moreinformative for resolving taxonomic questions in M. avidus species complex compared to the mtDNA marker. Moreover, the allozymes confirmed different seasonal preferences of the two taxa when they exist at the same locality. Since the studies of taxonomically diverse and challenging taxa are important for identification of areas of genetic endemism, we highly recommend integrative usage of allozyme and morphological markers. | URI: | https://open.uns.ac.rs/handle/123456789/7673 | ISSN: | 03240770 |
Appears in Collections: | Naučne i umetničke publikacije |
Show full item record
SCOPUSTM
Citations
7
checked on Feb 22, 2020
Page view(s)
10
Last Week
8
8
Last month
0
0
checked on May 10, 2024
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.