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https://open.uns.ac.rs/handle/123456789/7304
Назив: | Phenol removal from aqueous solutions by peroxidase extracted from horseradish | Аутори: | Savić, M. Stojmenović, D. Petronijević M. Petronijević Z. |
Датум издавања: | 1-мар-2014 | Часопис: | Applied Biochemistry and Microbiology | Сажетак: | Horseradish peroxidase (HRP) is one of the most recently used enzymes in the process of enzymatic phenol removal. It has a catalytic ability over a broad range of pH, temperature and contaminant concentrations. In this study we revealed the possibility of successful use the crude peroxidase obtained from horseradish roots for the phenol removal from aqueous solutions in the presence of the low molecular polyethylene glycol (PEG 300) at room temperature (20°C) and pH 7.2. Reaction was monitored by direct measuring of the absorbance changes in a samples taken at certain time intervals from the reaction mixture. At the first time PEG 300 was shown to be a more stabilizing effect on crude HRP and provided a higher phenol removal in comparison with PEG 3350. Crude HRP used in these study demonstrated a greater resistance on phenol and hydrogen peroxide inactivation that allowed a higher phenol removal. The highest phenol removal was achieved when the concentration of PEG 300, phenol and hydrogen peroxide were 300 mg/L, 2.0 and 2.5 mM, respectively. © 2014 Pleiades Publishing, Inc. | URI: | https://open.uns.ac.rs/handle/123456789/7304 | ISSN: | 00036838 | DOI: | 10.1134/S0003683814020161 |
Налази се у колекцијама: | Naučne i umetničke publikacije |
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