Dissipation studies of acetamiprid in apples under field conditions

Abstract

© 2019 International Society for Horticultural Science. All rights reserved. In this study, acetamiprid was applied for control of Cydia pomonella in concentration of 0.025%, in BBCH 75 apple development phase. The field trials were carried out in the orchard near Novi Sad, Serbia. The trials were designed according to standard OEPP methods for experimental design and data analysis. In Serbia, the MRL of acetamiprid residue in apple is 0.8 mg kg-1, with 28 days preharvest interval. Sampling was performed by random collection from various places of the experimental plots according to the FAO/WHO recommendations. Samples were collected immediately before and after acetamiprid application (when the spraying mixture has dried), 7, 14, 21, 28 days after the application and at harvest time. The untreated apple trees were used as blank samples, for the study of the matrix effects and to the recovery studies. For the determination of acetamprid residues, the QuEChERS method coupled with HPLC/DAD was carried out. The method was validated in accordance with SANCO/12571/2013 criteria (SANCO, 2013). The results showed that the average recovery at the four concentration levels was 96.45±2.3%; limit of quantification was established at 0.01 mg kg-1; within-laboratory reproducibility of 1.59%. Matrix-effect did not significantly affect the response of acetamiprid. Content of acetamiprid in apple samples after preharvest interval (PHI) were below MRL. Under field conditions the half-life value of acetamprid was 4.076 days.