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https://open.uns.ac.rs/handle/123456789/5232
Title: | Polypyrrole-based enzyme electrode with immobilized glucose oxidase for electrochemical determination of glucose | Authors: | Jugović, Branimir Grgur, Branimir Antov, Mirjana Knežević-Jugović, Zorica Stevanović, Jamina Gvozdenović, Milica |
Issue Date: | 2016 | Publisher: | Elsevier | Journal: | International Journal of Electrochemical Science | Abstract: | Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrrole at constant current density of 2 mA cm-2. Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm-2 for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm-3 which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. | URI: | https://open.uns.ac.rs/handle/123456789/5232 | ISSN: | 14523981 | DOI: | https://doi.org/10.1016/S1452-3981(23)15912-8 |
Appears in Collections: | TF Publikacije/Publications |
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