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https://open.uns.ac.rs/handle/123456789/4803
Title: | Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction | Authors: | Antov, Mirjana Ivetić, Darjana Knežević Jugović, Zorica |
Issue Date: | 3-Mar-2016 | Publisher: | Taylor & Francis Group | Journal: | Separation Science and Technology (Philadelphia) | Abstract: | © 2016 Taylor & Francis. Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w−1) polyethylene glycol 4000/15% (w w−1) KH2PO4/70% (w w−1) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery. | URI: | https://open.uns.ac.rs/handle/123456789/4803 | ISSN: | 01496395 | DOI: | 10.1080/01496395.2015.1117106 |
Appears in Collections: | TF Publikacije/Publications |
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