Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/4803
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Antov, Mirjana | en_US |
dc.contributor.author | Ivetić, Darjana | en_US |
dc.contributor.author | Knežević Jugović, Zorica | en_US |
dc.date.accessioned | 2019-09-30T08:41:58Z | - |
dc.date.available | 2019-09-30T08:41:58Z | - |
dc.date.issued | 2016-03-03 | - |
dc.identifier.issn | 01496395 | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/4803 | - |
dc.description.abstract | © 2016 Taylor & Francis. Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w−1) polyethylene glycol 4000/15% (w w−1) KH2PO4/70% (w w−1) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Taylor & Francis Group | en_US |
dc.relation.ispartof | Separation Science and Technology (Philadelphia) | en_US |
dc.title | Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.doi | 10.1080/01496395.2015.1117106 | - |
dc.identifier.scopus | 2-s2.0-84958779524 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84958779524 | - |
dc.description.version | Published | en_US |
dc.relation.lastpage | 628 | en_US |
dc.relation.firstpage | 622 | en_US |
dc.relation.issue | 4 | en_US |
dc.relation.volume | 51 | en_US |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Tehnološki fakultet, Katedra za primenjene i inženjerske hemije | - |
crisitem.author.orcid | 0000-0002-2160-6023 | - |
crisitem.author.parentorg | Tehnološki fakultet | - |
Appears in Collections: | TF Publikacije/Publications |
SCOPUSTM
Citations
3
checked on May 3, 2024
Page view(s)
28
Last Week
8
8
Last month
0
0
checked on May 10, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.