Supercritical fluid extraction of coriander seeds: Process optimization, chemical profile and antioxidant activity of lipid extracts

Abstract

© 2016 Elsevier B.V. Coriander seeds have been used in traditional medicine for the treatment of various ailments. Taking into account that operational conditions may influence the chemical composition optimization of supercritical extraction was performed. Influence of three different extraction parameters (pressure, temperature and CO2 flow) was explored while optimization of the process was performed by using Box-Behnken experimental design (BBD) in combination with response surface methodology (RSM). Experimental results were fitted to a second-order polynomial model with multiple regression, while analysis of variance (ANOVA) was employed in order to assess model fitness and determine optimal conditions for extraction yield (199.50 bar, 40.15 °C, 0.396 kg/h of CO2). Investigation of chemical composition showed that linalool was the most abundant compound in all samples, followed by camphor, methyl chavicol, (+)-limonene, eucalyptol, eugenol, geraniol, γ-terpinene and α-terpineol. Results showed that 100 bar was the best pressure for isolation of monoterpenes, while temperature and CO2 flow rate values differ with the structure of desired monoterpene class. Furthermore, antioxidant activity of obtained extracts was determined using two different assays. Obtained results showed that observed extracts exhibit higher activity against lipid peroxidation, than against DPPH radical with the lowest inhibitory concentrations of 0.101 mg/mL and 2.364 mg/mL, respectively.