Biomarkers of oxidative stress in pregnant women with recurrent miscarriages

Abstract

© 2019 Walter de Gruyter GmbH, Berlin/Boston. The aim of this study was to evaluate the markers of oxidative stress (OS), as well as the magnesium (Mg) concentration, pregnancy-associated plasma protein A (PAPP-A) and human chorionic gonadotropin (β-hCG) values in patients with previous recurrent miscarriages, with the purpose of comparing the level of OS with normal pregnancy. The study was conducted as a prospective study, which included a total of 95 pregnant women divided into two groups. In the study group (n = 35), there were women with at least three consecutive previous miscarriages. The control group (n = 60) consisted of healthy pregnant women without previous miscarriages and complications in pregnancy. Blood samples were taken between the 11th and 14th weeks of gestation, and the values of superoxide dismutase (SOD-1), glutathione peroxidase (GSH-Px) and total antioxidant status (TAS) in the serum, as well as the Mg concentration, PAPP-A and β-hCG values were determined. The values of SOD and GHS-Px were statistically higher in the study group, while the values of TAS were statistically significantly higher in the control group. The values of the Mg concentration were statistically significantly higher in the control group, while the values of β-hCG were statistically significantly higher in the study group. When the study group was divided into subgroups according to the outcome of pregnancy, there were statistical differences in the level of GSH-Px, SOD between miscarriages and the term delivery group, as well as in the Mg concentration between miscarriages and the term delivery group and between the term and preterm delivery groups. Higher values of SOD and GHS-Px, and lower values of TAS and Mg concentration could be used as markers for the prediction of spontaneous miscarriages. The results of this research indicated higher OS and exhaustion of antioxidant mechanisms in early pregnancy in a group with recurrent miscarriages. Also, lower values of serum Mg concentration could be one of the markers for the prediction of spontaneous miscarriages.