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https://open.uns.ac.rs/handle/123456789/3461
Nаziv: | Validation and application of FTIR spectroscopy in raw milk analysis | Аutоri: | Kučević, Denis Glamočić, Dragan Trivunović, Dario Jajić, Igor Perišić B. Krstović, Saša |
Dаtum izdаvаnjа: | 1-јан-2017 | Čаsоpis: | Acta Periodica Technologica | Sažetak: | The aim of this study was to investigate whether FTIR spectroscopy is an accurate and valid technique for the assessment of quality parameters in raw cow's milk: fat, protein, lactose, and total solids. The assessment was based on calibration series and comparison with reference material. Furthermore, it takes into account the results obtained in the inter-laboratory comparisons (proficiency testing). The calibration samples were purchased from the accredited regional reference laboratories. The validation parameters included linearity, accuracy, repeatability, reproducibility, and robustness. The linearity ratio was 0.95%. The biases calculated for the fat, protein, lactose and dry matter were -0.33, 0.31, -0.25, and 0.06 respectively. The F value from the F-test was used to determine the significant differences between two independent sets of the results. The obtained results were as follows: 1.469 for fat, 1.634 for protein, 1.192 for lactose, and 0.528 for dry matter. The intra-laboratory reproducibility calculated as the Horwitz Ratios for all parameters were within the criterion limits (0.5 to 0.8). The data obtained for carry-over were 0.27% for fat, 0.52% for protein, 0.47% for lactose, and 0.47% for dry matter. Based on the obtained results it can be concluded that the FTIR spectroscopy is a reliable instrumental technique for the determination of fat, protein, lactose and total solids in raw cow's milk. | URI: | https://open.uns.ac.rs/handle/123456789/3461 | ISSN: | 14507188 | DOI: | 10.2298/APT1748167K |
Nаlаzi sе u kоlеkciјаmа: | POLJF Publikacije/Publications |
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