Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/3453
Title: | Optimization of the medium composition for production of antimicrobial substances by Bacillus subtilis atcc 6633 | Authors: | Rončević, Zorana Đuran, Jovana Grahovac (Ranković), Jovana Dodić, Siniša Ranitović (Velićanski), Aleksandra Dodić, Jelena |
Issue Date: | 2017 | Publisher: | Novi Sad: University of Novi Sad, Faculty of Technology, Novi Sad | Journal: | Acta Periodica Technologica | Abstract: | In the effort to overcome the increase in antimicrobial resistance of different pathogens, natural products from microbial sources appear to be the most favorable alternative to current antibiotics. Production of antimicrobial compounds is highly dependent on the nutritional conditions. Hence, in order to achieve high product yields, selection of the media constituents and optimization of their concentrations are required. In this research, the possibility of antimicrobial substances production using Bacillus subtilis ATCC 6633 was investigated. Also, optimization of the cultivation medium composition in terms of contents of glycerol, sodium nitrite and phosphates was done. Response surface methodology and the method of desirability function were applied for determination of optimal values of the examined factors. The developed model predicts that the maximum inhibition zone diameters for Bacillus cereus ATCC 10876 (33.50 mm) and Pseudomonas aeruginosa ATCC 27853 (12.00 mm) are achieved when the initial contents of glycerol, sodium nitrite and phosphates were 43.72 g/L, 1.93 g/L and 5.64 g/L, respectively. The results of these experiments suggest that further research should include the utilization of crude glycerol as a carbon source and optimization of composition of such media and cultivation conditions in order to improve production of antimicrobial substances using Bacillus subtilis ATCC 6633. | URI: | https://open.uns.ac.rs/handle/123456789/3453 | ISSN: | 14507188 | DOI: | 10.2298/APT1748245R |
Appears in Collections: | TF Publikacije/Publications |
Show full item record
SCOPUSTM
Citations
1
checked on May 10, 2024
Page view(s)
27
Last Week
7
7
Last month
0
0
checked on May 10, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.