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https://open.uns.ac.rs/handle/123456789/32709
Поље DC-а | Вредност | Језик |
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dc.contributor.author | Jarić, Stefan | en_US |
dc.contributor.author | Kudriavtseva, Anastasiia | en_US |
dc.contributor.author | Nekrasov, Nikita | en_US |
dc.contributor.author | Orlov, Alexey | en_US |
dc.contributor.author | Komarov, Ivan | en_US |
dc.contributor.author | Barsukov, Leonty | en_US |
dc.contributor.author | Gađanski, Ivana | en_US |
dc.contributor.author | Nikitin, Petr | en_US |
dc.contributor.author | Bobrinetskiy, Ivan | en_US |
dc.date.accessioned | 2024-04-25T10:25:10Z | - |
dc.date.available | 2024-04-25T10:25:10Z | - |
dc.date.issued | 2024-01 | - |
dc.identifier.issn | 0026-265X | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/32709 | - |
dc.description.abstract | Measuring NT-proBNP biomarker is recommended for preliminary diagnostics of the heart failure. Recent studies suggest a possibility of early screening of biomarkers in saliva for non-invasive identification of cardiac diseases at the point-of-care. However, NT-proBNP concentrations in saliva can be thousand time lower than in blood plasma, going down to pg/mL level. To reach this level, we developed a label-free aptasensor based on a liquid-gated field effect transistor using a film of reduced graphene oxide monolayer (rGO-FET) with immobilized NT-proBNP specific aptamer. We found that, depending on ionic strength of tested solutions, there were different levels of correlation in responses of electrical parameters of the rGO-FET aptasensor, namely, the Dirac point shift and transconductance change. The correlation in response to NT-proBNP was high for 1.6 mM phosphate-buffered saline (PBS) and zero for 16 mM PBS in a wide range of analyte concentrations, varied from 1 fg/mL to 10 ng/mL. The effects of transconductance and Dirac point shift in PBS solutions of different concentrations are discussed. The biosensor exhibited a high sensitivity for both transconductance (2 µS/decade) and Dirac point shift (2.3 mV/decade) in diluted PBS with the linear range from 10 fg/mL to 1 pg/mL. The aptasensor performance has been also demonstrated in undiluted artificial saliva with the achieved limit of detection down to 41 fg/mL (∼4.6 fM). | en_US |
dc.publisher | Elsevier | en_US |
dc.relation | Antares | en_US |
dc.relation.ispartof | Microchemical Journal | en_US |
dc.title | Femtomolar detection of the heart failure biomarker NT-proBNP in artificial saliva using an immersible liquid-gated aptasensor with reduced graphene oxide | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.microc.2023.109611 | - |
dc.description.version | Published | en_US |
dc.relation.lastpage | 16 | en_US |
dc.relation.firstpage | 1 | en_US |
dc.relation.volume | 196 | en_US |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
crisitem.project.projectURL | https://biosens.rs/en/projects/centre-of-excellence-for-advanced-technologies-in-sustainable-agriculture-and-food-security-2 | - |
crisitem.author.dept | Institut BioSense | - |
crisitem.author.dept | Institut BioSense | - |
crisitem.author.dept | Institut BioSense | - |
crisitem.author.orcid | 0000-0001-7933-3542 | - |
crisitem.author.orcid | 0000-0003-0713-7897 | - |
crisitem.author.parentorg | Univerzitet u Novom Sadu | - |
crisitem.author.parentorg | Univerzitet u Novom Sadu | - |
crisitem.author.parentorg | Univerzitet u Novom Sadu | - |
Налази се у колекцијама: | IBS Publikacije/Publications |
Датотеке у овој ставци:
Датотека | Величина | Формат | |
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Femtomolar detection of the heart failure.pdf | 3.85 MB | Adobe PDF | Погледајте |
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