Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/32454
Title: | Visualisation of Ti3C2TX MXenes in eukaryotic cells by Transmission Electron Microscopy | Authors: | Sergiy Kyrylenko Peace Ekpe Yevheniia Husak Illya Yanko Anton Roshchupkin Andriy Stepanenko Oleksiy Gogotsi Veronika Zahorodna Daniil Burduli Ivan Baginskyi Vitalii Balitskyi Maksym Pogorielov |
Keywords: | MXenes;TEM | Issue Date: | 5-Sep-2021 | Publisher: | Sumy State University | Project: | H2020 SALSETH | Conference: | IEEE 11th International Conference on "Nanomaterials: Applications & Properties" (NAP-2021) | Abstract: | We used TEM metal grids with vacuum deposited carbon support films. However, traditionally used Cu grids were too toxic for the cells. We then attempted to grow cells on the carbon film sprayed on the plastic culture dish. We postulated that the pieces of such plastic along with the film with the fixed cells could be dissolved in chloroform, releasing the film with the firmly attached cells. We tried to dissolve the plastic on the interface of chloroform and water, with the idea that the carbon film would float to the water surface, where it could be caught by the grid. However, the dissolved plastic was forming thin layers on top of water and prevented catching the carbon films. We then tried to dissolve the plastic in chloroform only. It was indeed possible to locate the released film in the transmission light and catch it with the grids. However, such approach gave unsatisfactory results due to intensive jamming of the carbon films with the cells. | URI: | https://open.uns.ac.rs/handle/123456789/32454 | Rights: | Attribution-NonCommercial-NoDerivs 3.0 United States |
Appears in Collections: | Projekti |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Peace TEM NAP2021 Abstract fin.pdf | 110.24 kB | Adobe PDF | View/Open |
Page view(s)
28
Last Week
4
4
Last month
0
0
checked on May 10, 2024
Download(s)
12
checked on May 10, 2024
Google ScholarTM
Check
This item is licensed under a Creative Commons License