Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells

Abstract

© 2015, Association of Chemists and Chemical Engineers of Serbia. All rights reserved. Fullerenol (C<inf>60</inf>(OH)<inf>24</inf>) nanoparticles (FNP) have a significant role in biomedical research due to their numerous biological activities, some of which have cytoprotective and antioxidative properties. The aim of this study was to measure distribution of fullerenol nanoparticles and zeta potential in cell medium RPMI 1640 with 10% fetal bovine serum (FBS) and to investigate the influence of FNP on Chinese hamster ovary cells (CHO-K1) survival, as well as to determine the activity of three antioxidative enzymes: superoxide-dismutase, glutathione-reductase and glutathione-S-transferase in mitomycin C-treated cell line. Our investigation implies that FNP, as a strong antioxidant, influences the cellular redox state and enzyme activities and thus may reduce cell proliferation, which confirms that FNP could be exploited for its use as a cytoprotective agent.