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https://open.uns.ac.rs/handle/123456789/28227| Title: | Antiradical and antiproliferative activity of selected plantextracts from Rosaceae and Ericaceae family Antiradikalska i antiproliferativna aktivnost ekstrakata odabranih biljaka iz familija rosaceae i ericaceae |
Authors: | Tumbas Vesna | Keywords: | Berries, antiradical activity, antiproliferative activity, polyphenoliccompounds, vitamin C, superoxide anion radicals,hydroxyl radicals, DPPH●, HPLC, ESR, SPE;Bobičasto voće, antiradikalska aktivnost, antiproliferativna aktivnost, polifenolna jedinjenja, vitamin C, superoksid anjon radikali, hidroksil radikali, DPPH●, HPLC, ESR, SPE | Issue Date: | 14-Jun-2010 | Publisher: | Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad University of Novi Sad, Faculty of Technology at Novi Sad |
Abstract: | <p>Acetonski ekstrakti bobičastog voća iz familija Ericaceae<br />(borovnica,<em> Vaccinium myrtillus</em> L., i brusnica, <em>Vaccinium<br />macrocarpon</em> L.) i Rosaceae (šipak, <em>Rosa canina</em> L., i glog,<br /><em>Crataegus oxyacantha</em> L.) prečišćeni su i frakcionisani<br />primenom ekstrakcije na čvrstoj fazi (SPE). Sadržaj ukupnih<br />polifenolnih jedinjenja, flavonoida i antocijana u prečišćenim<br />ekstraktima određeni su spektrofotometrijskim metodama.<br />HPLC analizom utvrđen je kvalitativni i kvantitativni<br />sastav frakcija ekstrakata ispitivanih bobica. ESR spektroskopijom<br />ispitana je antiradikalska aktivnost frakcija ekstrakata<br />bobica na stabilne DPPH<sup>●</sup> i reaktivne superoksid anjon i<br />hidroksil radikale. ESR spektroskopijom ispitano je i<br />prisustvo slobodnih radikala antioksidanata nastalih tokom<br />reakcije frakcija ekstrakata bobica sa superoksid anjon<br />radikalima. U završnoj fazi rada ispitana je<em> in vitro</em><br />antiproliferativna aktivnost frakcija ekstrakata bobica, njihovim<br />delovanjem na rast tri histološki različite humane ćelijske<br />linije: HeLa (epitelni karcinom cerviksa), HT-29 (adenokarcinom<br />debelog creva) i MCF-7 (adenokarcinom dojke).</p> <p>Acetone extracts of berries form Ericaceae (bilberry, <em>Vaccinium<br />myrtillus</em> L., and cranberry, <em>Vaccinium macrocarpon</em><br />L.) and Rosaceae (rose hip, <em>Rosa canina</em> L., and hawthorn,<br /><em>Crataegus oxyacantha </em>L.) families were purified and fractionated<br />using solid phase extraction (SPE). Contents of total<br />polyphenols, flavonoids and anthocyanins in purified extracts<br />were determined by spectrophotometric methods.<br />HPLC analysis were used for quantitative and qualitative<br />characterization of investigated berry extracts fractions. ESR<br />spectroscopy was used for investigation of antiradical activity<br />of berry extracts fractions on stable DPPH<sup>●</sup> and<br />reactive superoxide anion and hydroxyl radicals. The<br />presence of antioxidant free radicals formed during reaction<br />of investigated berry extracts fractions with superoxide<br />anion radicals was also investigated by ESR. Antiprolixferative<br />activity of investigated berry extracts fractions was<br />determined<em> in vitro</em>, testing their influence on the growth of<br />three histologically different human cell lines: HeLa (cervix<br />epithelioid carcinoma), HT-29 (colon adenocarcinoma) i<br />MCF-7 (breast adenocarcinoma).</p> |
URI: | https://open.uns.ac.rs/handle/123456789/28227 |
| Appears in Collections: | TF Teze/Theses |
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