Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/27118
Title: | Antioxidant properties of Galium verum L. (Rubiaceae) extracts | Authors: | Lakić Neda Mimica-Dukić Neda Isak Jelena Bozin Biljana |
Issue Date: | 2010 | Journal: | Central European Journal of Biology | Abstract: | The antioxidant properties of methanol extracts of Lady’s Bedstraw (Galium verum L., Rubiaceae) herb from two different localities in Serbia were evaluated. Antioxidant activity was assessed in four different model systems. Free radical scavenging capacity (RSC) was examined by measuring the scavenging activity of extracts on 2,2-diphenyl-1-pycrylhydrazil (DPPH) and hydroxyl radical (OH), as well as on hydrogen peroxide. In addition, the protective effects of lipid peroxidation (LP) in corn oil were evaluated by the TBA-assay using the Fe2+/ascorbate system of induction. The amount of dried extract, the content of total phenolics, flavonoids and chlorophylls was also determined. Extracts from both locations expressed very strong scavenger activity, reducing the DPPH⊙ (IC50=3.10 µg/mland 8.04 µg/ml) and OH radical formation (IC50=0.05 µg/ml and 0.54 µg/ml) and neutralising H2O2 (IC50=4.98 µg/ml and 3.80 µg/ml), in a dose dependant manner. Also, examined extracts showed notable inhibition of LP (IC50=11.69 µg/ml and 19.47 µg/ml). The observed differences in antioxidant activity could be partially explained by the levels of phenolics (2.44–4.65 mg and 4.57–5.16 mg gallic acid equivalents/g dry extract), flavonoids (6.38–10.70 µg and 15.56–17.96 µg quercetin equivalents/g dry extract) and chlorophylls in the investigated Lady’s Bedstraw extracts. | URI: | https://open.uns.ac.rs/handle/123456789/27118 | ISSN: | 1895-104X 1644-3632 |
Appears in Collections: | MDF Publikacije/Publications |
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.