Primena Pseudomonas aeruginosa bakteriofaga kao antimikrobnih agenasa

Abstract

<p>Cilj rada bio je da se utvrdi da li se bakteriofagi mogu primenjivati kao antimikrobni agensi, s obzirom da je reč o virusima koji liziraju bakterijske ćelije. U radu su prikazani rezultati izolacije <strong>Pseudomonas aeruginosa</strong>&nbsp;specifičnih faga iz različitih prirodnih sredina i ispitana je mogućnost njihove primene kao antimikrobnih agenasa. U tu svrhu ispitane su morfolo&scaron;ke karakteristike odabranih faga, karakterisike proteinskog profila, genoma i potencijalnih receptora.Takođe, ispitana je i adsorpcija i stabilnost faga u različitim uslovima sredine, kao i pojava mutnata bakterija rezistentnih na fage. Primenom kolorimetrijskih metoda u mikrotitar pločama ispitana je litička efikasnost odabranih faga, tačnije njihov efekat na rast bakterije, formiranje biofilma i efekat na već formirani biofilm. U radu je ispitana i mogućnosti kombinovanja bakteriofaga i antibiotika, pri čemu je utvrđen sinergizam svih faga i ceftriaksona. Na osonovu dobijenih rezultata jasno je da bakteriofagi imaju izuzetan potencijal kao alternativni agensi u kontroli multiplo rezistentnih sojeva <strong>P. aeruginosa</strong>.</p>

<p>The aim of the study was to determine whether the phages could be<br />used as antimicrobial agents, as they are viruses capable to lyse<br />bacterial cells. In the paper, the results of Pseudomonas specific<br />phage isolation from various environments were presented, and their<br />antimicrobial potetnial was determined. Accordingly, properties of<br />selected phages were examined- morphology, protein profiles,<br />characteristics of genome and potential receptors. In addition,<br />adsorption and stability of phages in various environmental<br />conditions were examined, as well as appearance of bacterial<br />mutants resistant to phages. The lytic efficacy of phages- their<br />growth and biofilm formation inhibition, as well as effect on already<br />formed biofilm was determined using the colorimetric microtitre<br />plate methods. The possible combinations of phages and antibiotics<br />were also examined, and synergy among all phages and ceftriaxone<br />was observed. According to the results, it is obvious that the phages<br />have a great potential to be used as alternative antimicrobial agents<br />to combat multiple resistant P. aeruginosa.</p>