Impact of the fibroblast morphogenetic field information on human healthy and tumor cell lines in vitro

Abstract

© Nova Science Publishers, Inc. Biological investigation was conducted to study in vitro changes in morphological properties and metabolic activity of human continuous cell lines after treatment with the morphogenetic field hologram information (the field). We used human tumor cell lines, including cervical (HeLa) and lung cancer (A549), as well as one normal fetal lung fibroblasts cell line (MRC-5) to investigate the effect of such treatment. Metabolic activity of investigated cell lines was evaluated by MTT assay. Results showed that MRC-5 cells exposed 3 h to the field had 50% increases in metabolic activity 48 h after treatment in comparison to untreated control cells. Similar but lower effect was observed in both tested tumor cell lines. Changes in cells depended also on the exposure time. Morphology of the cells was examined by visual observations using light microscopy. Metabolic activity was evaluated by MTT assay. Results of this study showed that application of the field has measurable influence on biological systems.