Role of bacteriophages from family Siphoviridae in lysogenic conversion of Bordetella bronchiseptica and their antimicrobial potential

Abstract

<p>U ovom radu su izolovani specifičn<em>i&nbsp; Bordetella bronchiseptica</em>&nbsp; bakteriofagi iz iz<br />priodne sredine koji pripadaju familiji&nbsp; <em>Siphoviridae</em>. Bakteriofagi su&nbsp; okarakterisani u<br />cilju razmatranja njihove uloge u lizogenoj konverziji bakterije i mogućnosti<br />primene u kontroli vrste&nbsp;<em> B. bronchiseptica</em>. U tu svrhu ispitivane su morfolo&scaron;ke<br />karakteristike odabranih faga, litički&nbsp; spektar faga, i karakteristike genoma i proteina<br />faga. Takođe, ispitivana je i stabilnost faga u različitim uslovima sredine, njihova<br />litička efikasnost i efekat na formiranje i vec formirani biofilm. U radu je ispitana i<br />uloga faga u produkciji biofilma,&nbsp; rezistenciji na antibiotike, procesu hemolize i<br />pokretljivosti kod vrste&nbsp; <em>B. bronchiseptica</em>.&nbsp; Rezultati ovog rada jasno ukazuju na<br />ulogu faga u lizogenoj konverziji vrste&nbsp;<em> B.&nbsp; bronchiseptica</em>&nbsp; kao&nbsp; i mogućnost njihove<br />primene, uz određene modifikacije, kao anti-<em>B.bronchiseptica</em> agenasa.</p>

<p>In this paper <em>Bordetella bronchiseptica</em> specific phages belonging to family <em>Siphoviridae</em> were isolated from environment. Bacteriophages were characterized to determine their role in the lysogenic conversion of bacteria and their possible usage in <em>B. bronchiseptica </em>control. For this purpose, morphological characteristics of the selected phages, the phage lytic spectrum, and the characteristics of phage genome and the proteins were examined. Also, the stability of phage in different environmental conditions was studied as well as their lytic efficiency, effect on the biofilm formation and formed biofilm. The paper also examined the role of phages in the production of biofilm, resistance to antibiotics, the process of hem olysis and the motility of<em> B. bronchiseptica </em>species. The results of this study clearly indicate the role of phage in lysogenic conversion in <em>B. bronchiseptica</em> as well as their potential for <em>B. bronchiseptica</em> control.</p>