Evaluation of the lipophilicity of some dehydroepiandrosterone derivates using RP-18 HPTLC chromatography

Abstract

The Chromatographic behavior of DHEA derivates - 17α-substituted- 3β,17β-dihydroxy-16-oximino derivatives of 5-androstene has been studied by reversed-phase high-performance thin-layer chromatography. HPTLC was performed on a C-18 bonded phase with two aqueous eluents, acetone-water and dioxane-water. Linear relationship between the retention parameters (RM) and the organic modifier content in the mobile phase allows the extrapolation procedure. The influence of substituent in the molecule on extrapolated retention data is discussed. The correlation between the chromatographic lipophilic parameters (RMo) and the calculated log P values, as well as biological activity, has been studied. The results show that reversed-phase chromatographic (RMo) are useful in describing the lipophilic nature of investigated compounds as well as the activity.