Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/15526
Title: | Isoflavone composition, total polyphenolic content, and antioxidant activity in soybeans of different origin | Authors: | Vesna Tepavčević Milica Atanacković Krstonošić Jegor Miladinović Đorđe Malenčić Jovan Popović Jelena Helen Hogervorst |
Keywords: | 2,2-diphenyl-1-picrylhydrazyl assay;high-performance liquid chromatography;soybean;phytoestrogens | Issue Date: | 1-Jun-2010 | Journal: | Journal of Medicinal Food | Abstract: | Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P < .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized. © 2010, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition. | URI: | https://open.uns.ac.rs/handle/123456789/15526 | ISSN: | 1096620X | DOI: | 10.1089/jmf.2009.0050 |
Appears in Collections: | MDF Publikacije/Publications POLJF Publikacije/Publications |
Show full item record
SCOPUSTM
Citations
42
checked on May 3, 2024
Page view(s)
34
Last Week
9
9
Last month
0
0
checked on May 3, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.