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https://open.uns.ac.rs/handle/123456789/15135
Nаziv: | C-terminal domain of chromogranin B regulates intracellular calcium signaling | Аutоri: | Schmidt S. Mo M. Heidrich F. Ćelić, Anđelka Ehrlich B. |
Dаtum izdаvаnjа: | 30-дец-2011 | Čаsоpis: | Journal of Biological Chemistry | Sažetak: | The versatility of intracellular calcium as a second messenger is seen in its ability to mediate opposing events such as neuronal cell growth and apoptosis. A leading hypothesis used to explain how calcium regulates such divergent signaling pathways is that molecules responsible for maintaining calcium homeostasis have multiple roles. For example, chromogranin B (CGB), a calcium binding protein found in secretory granules and in the lumen of the endoplasmic reticulum, buffers calcium and also binds to and amplifies the activity of the inositol 1,4,5-trisphosphate receptor (InsP 3R). Previous studies have identified two conserved domains of CGB, an N-terminal domain (N-CGB) and a C-terminal domain (C-CGB). N-CGB binds to the third intraluminal loop of the InsP 3R and inhibits binding of full-length CGB. This displacement of CGB decreases InsP 3R-dependent calcium release and alters normal signaling patterns. In the present study, we further characterized the role of N-CGB and identified roles for C-CGB. The effect of N-CGB on calcium release depended upon endogenous levels of cellular CGB, whereas the regulatory effect of C-CGB was apparent regardless of endogenous levels of CGB. When either full-length CGB or C-CGB was expressed in cells, calcium transients were increased. Additionally, the calcium signal initiation site was altered upon C-CGB expression in neuronally differentiated PC12 and SHSY5Y cells. These results show that CGB has numerous regulatory roles and that CGB is a critical component in modulating InsP 3R-dependent calcium signaling. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. | URI: | https://open.uns.ac.rs/handle/123456789/15135 | ISSN: | 00219258 | DOI: | 10.1074/jbc.M111.251330 |
Nаlаzi sе u kоlеkciјаmа: | PMF Publikacije/Publications |
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