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https://open.uns.ac.rs/handle/123456789/14000
Title: | Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus | Authors: | Antov, Mirjana Peričin, Draginja Dašić, Milica |
Issue Date: | Jan-2006 | Publisher: | Elsevier | Journal: | Process Biochemistry | Abstract: | The partitioning of xylanase, produced by solid-state cultivation of Polyporus squamosus, in aqueous two-phase system polyethylene glycol/ammonium sulphate was studied. In system 10% (w/w) polyethylene glycol 1500/20% (w/w) ammonium sulphate/70% (w/w) crude enzyme at pH 5.1 favourable partition coefficient and the top phase yield were obtained, 85.6% and 97.37%, respectively, followed by purification factor 4.8. This result suggests the possible integration of cultivation and primary downstream processing step by partitioning of xylanase in aqueous two-phase system. © 2005 Elsevier Ltd. All rights reserved. | URI: | https://open.uns.ac.rs/handle/123456789/14000 | ISSN: | 13595113 | DOI: | 10.1016/j.procbio.2005.06.010 |
Appears in Collections: | TF Publikacije/Publications |
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