Normal and reversed phase thin layer chromatography of selected 2,5- anhydroaldohexose ethylene acetal derivatives

Abstract

The chromatographic behavior of 31 samples of variously substituted 2,5- anhydroaldohexose ethylene acetal derivatives has been studied on silica gel and C-18 modified silica gel layers with, respectively, binary non-aqueous and aqueous mobile phases. The slopes and intercepts of the linear relationships between the retention constant (R(M)) and the logarithm of the volume fraction of the diluent in non-aqueous mobile phase, as well as of the volume fraction of organic component in aqueous-organic mobile phase, have been calculated and are discussed in relation to solute and mobile and stationary phase characteristics. The retention and relative retention of compounds depend largely on the retention behavior of their substituents.