Toward consistent discrimination of common bean (Phaseolus vulgaris L.) based on grain coat color, phytochemical composition, and antioxidant activity

Abstract

© 2019 Wiley Periodicals, Inc. In this paper, 52 common bean (Phaseolus vulgaris L.) genotypes were analyzed regarding their phytochemical composition and antioxidant activity, as well as other characteristics (grain coat color, grain size, and growth size). The total phenols content (TPC), determined according to the Folin–Ciocalteu method, varied from 0.166 to 0.772 mg/100 g DM, while total flavonoids content (TFC), determined by aluminum chloride colorimetric assay, varied from 4.861 to 161.861 mg/100 g DM. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity and ranged from 1.419 to 12.917 mg/ml. The antioxidant activity was predicted using multiple linear regression (MLR). The obtained results revealed certain influence of grain coat color on common bean phytochemical composition and antioxidant activity that was confirmed throughout classification method—principal component analysis (PCA). White grain coat color samples were distinguished from colored ones on the basis of their phytochemical composition and antioxidant activity. Practical applications: The antioxidant activity of 52 common bean (Phaseolus vulgaris L.) genotypes was predicted using MLR. A certain influence of grain coat color on common bean phytochemical composition and antioxidant activity was detected applying classification chemometric techniques.