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https://open.uns.ac.rs/handle/123456789/11398
Title: | Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts | Authors: | Stanojević, Ljiljana Stanković, Mihajlo Nikolić, Vesna Nikolić, Ljubiša Ristić, Dušica Čanadanović-Brunet, Jasna Tumbas Šaponjac, Vesna |
Issue Date: | 16-Jul-2009 | Publisher: | MDPI | Journal: | Sensors | Abstract: | The antioxidant activity of water, ethanol and methanol Hieracium pilosella L. extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH• free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin- Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH• radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59-244.16 mg GAE/g of dry extract) and total flavonoids (79.13-82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity. © 2009 by the authors. | URI: | https://open.uns.ac.rs/handle/123456789/11398 | ISSN: | 14248220 | DOI: | 10.3390/s90705702 |
Appears in Collections: | FF Publikacije/Publications TF Publikacije/Publications |
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