Modulation of TNF-α activity in tumor PC cells using anti-CD45 and anti-CD95 monoclonal antibodies

Abstract

TNF-α is a pleiotropic cytokine produced by activated T-cytotoxic lymphocytes and NK cells that is involved in signal transduction after interacting with the appropriate cell surface receptors. The modulation of signals by TNF-α receptor super-family is involved in the regulation of cell activation, proliferation, differentiation and control of the cell survival including cell death by apoptosis and necrosis. We have monitored the kinetics of apoptosis/necrosis on PC cells, after TNF-α exposure of pre-treated cells to anti-CD95 and anti-CD45 monoclonal antibodies. The results showed that in comparison with untreated cells, TNF-α, after 6-24 h of incubation significantly increased apoptosis and necrosis in PC cells. These effects were significantly different in comparison to both untreated cells and cells pre-treated with anti-CD45 monoclonal antibodies. However, TNF-α on PC cells pre-treated with anti-CD95 monoclonal antibody significantly decreased apoptotic and necrotic form of cell death. We concluded that anti-CD45 and CD95 monoclonal antibodies modulates the effect of TNF-α on this cell line in vitro, and that these molecules participate in TNF-α cytotoxic response. © 2004 Elsevier Ireland Ltd. All rights reserved.