Applying multivariate methods in the estimation of bioactivity properties of acetamide derivatives

Abstract

© Akadémiai Kiadó, Budapest. It has been established that the selected acetamide derivatives fulfill Veber's and Pardridge's rule of good bioavailability. The lipophilicity as a crucial molecular descriptor of biological activity for acetamide derivatives was determined by reversed-phase thinlayer chromatography (RP-TLC) in different mixtures of water and organic modifiers (ethanol and tetrahydrofuran). Also, lipophilicity was examined mathematically, by using relevant software packages. The effects of the substituent on the lipophilicity of acetamide derivatives were analyzed. By applying linear regression analysis and 2 multivariate methods (cluster analysis and principal component analysis), the obtained chromatographic parameters, RM0 and m, of the examined acetamides were correlated with the standard measure of lipophilicity, log P, important pharmacokinetic predictors, and selected toxicity parameters. The obtained results confirmed that chromatographic parameters, RM 0 and m, determined by RP-TLC, could be successfully used for describing the lipophilicity and estimation of pharmacokinetics and the toxic effects of the studied acetamide derivatives.