Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/9902
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leskovac V. | en |
dc.contributor.author | Peričin D. | en |
dc.contributor.author | Trivić S. | en |
dc.date.accessioned | 2020-03-03T14:35:48Z | - |
dc.date.available | 2020-03-03T14:35:48Z | - |
dc.date.issued | 1978-01-01 | en |
dc.identifier.issn | 0020711X | en |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/9902 | - |
dc.description.abstract | 1. 1. Commercial preparations of the enzyme are homogeneous on an ion-exchange column chromatography they are heterogeneous with respect to their zinc content, total -SH content, state of oxidation and the reactivity of their -SH groups. 2. 2. Heterogeneous properties of commercial preparations have not influenced their coenzyme binding capacity. All commercial preparations tested in this, and our earlier work (Leskovac & Pavkov-Peričin, 1975; Leskovac et al., 1976), bind approx. 2 molecules of NADH/tetrameric enzyme molecule (mol. wt. 144,000), in a non-cooperative fashion. We have arrived at this binding capacity by 3 different methods: fluorescent titration, gel-filtration and the rate enhancement of the Ellman reaction in the presence of coenzyme. 3. 3. pH-profile of the coenzyme dissociation constant indicates that 2 charged groups on the enzyme, with pK. 7.4 and 9.4, control the enzyme-coenzyme complex formation. 4. 4. Commercial preparations bind weakly coenzyme fragments, adenosyl pyrophosphate ribose or ADP, with a stoichiometry of 4-5 fragment molecules/tetrameric molecule of enzyme. 5. 5. After the binding, the coenzyme or its fragments induce a conformational change in the enzyme; the magnitude of this conformational change decreases in the following order: NADH + acetamide > NADH > adenosyl pyrophosphate ribose > ADP. © 1978. | en |
dc.relation.ispartof | International Journal of Biochemistry | en |
dc.title | Yeast alcohol dehydrogenase IV. Binding of reduced coenzyme and its fragments | en |
dc.type | Journal/Magazine Article | en |
dc.identifier.doi | 10.1016/0020-711X(78)90112-X | en |
dc.identifier.pmid | 9 | en |
dc.identifier.scopus | 2-s2.0-0017843689 | en |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/0017843689 | en |
dc.relation.lastpage | 371 | en |
dc.relation.firstpage | 365 | en |
dc.relation.issue | 5 | en |
dc.relation.volume | 9 | en |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | PMF Publikacije/Publications |
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